i-protease (Uhlobo olubhaliwe) Umkhiqizi we-Newgreen protease (Uhlobo olubhaliwe) Isengezo
Incazelo Yomkhiqizo
I-Protease yigama elivamile lesigaba sama-enzyme enza i-hydrolyze amaketanga e-protein peptide. Angahlukaniswa abe yi-endopeptidase kanye ne-telopeptidase ngokuya ngendlela ezehlisa ngayo ama-peptides. Owokuqala angasika iketango elikhulu le-polypeptide lesisindo samangqamuzana lisuka phakathi ukuze lakhe i-prion ne-peptone yamangqamuzana amancane; Ezokugcina zingahlukaniswa zibe i-carboxypeptidase ne-aminopeptidase, eyenza i-hydrolyze iketango le-peptide ngalinye lisuka ku-carboxyl yamahhala noma emaphethelweni e-amino e-polypeptide, ngokulandelana, kuya kuma-amino acid.
I-COA
| Izinto | Imininingwane | Imiphumela |
| Ukubukeka | Impushana Emhlophe | Impushana Emhlophe |
| Isivivinyo | ≥25u/ml | Dlula |
| Iphunga | Lutho | Lutho |
| Ukuminyana Okuxekethile(g/ml) | ≥0.2 | 0.26 |
| Ukulahlekelwa Ekumisweni | ≤8.0% | 4.51% |
| Izinsalela ekushiseni | ≤2.0% | 0.32% |
| PH | 5.0-7.5 | 6.3 |
| Isilinganiso sesisindo samangqamuzana | <1000 | 890 |
| Izinsimbi Ezisindayo(Pb) | ≤1PPM | Dlula |
| As | ≤0.5PPM | Dlula |
| Hg | ≤1PPM | Dlula |
| Ukubalwa Kwamagciwane | ≤1000cfu/g | Dlula |
| I-Colon Bacillus | ≤30MPN/100g | Dlula |
| Imvubelo & Isikhunta | ≤50cfu/g | Dlula |
| Amagciwane e-Pathogenic | Okubi | Okubi |
| Isiphetho | Vumelanisa nencazelo | |
| Impilo yeshelufu | Iminyaka emi-2 lapho igcinwe kahle | |
Umsebenzi
I-protease ikhona kakhulu ku-viscera yezilwane, iziqu zezitshalo, amaqabunga, izithelo kanye nama-microorganisms. Ama-microbial proteases akhiqizwa ikakhulukazi isikhunta namagciwane, alandelwe yiyeast kanye ne-actinomyces.
Ama-Enzymes enza i-hydrolysis yamaprotheni. Kunezinhlobo eziningi, ezibalulekile i-pepsin, i-trypsin, i-cathepsin, i-papain ne-subtilis protease. I-Protease inokukhetha okuqinile kwe-substrate yokusabela, futhi i-protease ingasebenza kuphela kwibhondi ethile ye-peptide ku-molecule yephrotheni, njengebhondi ye-peptide eyakhiwe yi-hydrolysis yama-amino acid ayisisekelo enziwa yi-trypsin. I-Protease isabalale kakhulu, ikakhulukazi emgudwini wokugaya ukudla womuntu nezilwane, futhi igcwele ezitshalweni nasezilwanyaneni ezincane. Ngenxa yezinsiza ezilinganiselwe zezilwane nezitshalo, ukukhiqizwa kwamalungiselelo e-protease embonini kwenziwa ikakhulukazi ngokuvutshelwa kwama-microorganisms afana ne-Bacillus subtilis ne-Aspergillus aspergillus.
Isicelo
I-Protease ingenye yamalungiselelo abaluleke kakhulu e-enzyme yezimboni, engakwazi ukuvuselela i-hydrolysis yamaprotheni kanye ne-polypeptide, futhi itholakala kakhulu ezithweni zezilwane, iziqu zezitshalo, amaqabunga, izithelo kanye nama-microorganisms. Ama-protease asetshenziswa kakhulu ekukhiqizeni ushizi, ekuthendeni kwenyama kanye nokuguqulwa kwamaprotheni ezitshalo. Ngaphezu kwalokho, i-pepsin, i-chymotrypsin, i-carboxypeptidase kanye ne-aminopeptidase zingama-protease emgudwini wokugaya ukudla komuntu, futhi ngaphansi kwesenzo sawo, iphrotheni efakwe emzimbeni womuntu i-hydrolyzed ibe ama-peptide amangqamuzana amancane nama-amino acid.
Njengamanje, ama-protease asetshenziswa embonini yokubhaka ama-fungal proteases, ama-bacterial proteases nama-plant proteases. Ukusetshenziswa kwe-protease ekukhiqizeni isinkwa kungashintsha izakhiwo ze-gluten, futhi indlela yayo yokusebenza ihlukile esenzweni samandla ekulungiseni isinkwa kanye nokusabela kwamakhemikhali kwe-ejenti yokunciphisa. Esikhundleni sokuphula isibopho se-disulfide, i-protease iphula inethiwekhi enezinhlangothi ezintathu eyenza i-gluten. Iqhaza le-protease ekukhiqizeni isinkwa libonakala ikakhulukazi ohlelweni lokuvutshelwa kwenhlama. Ngenxa yesenzo se-protease, iphrotheni ekufulawa iyancipha ibe ama-peptides nama-amino acid, ukuze inikeze umthombo we-yeast carbon futhi ikhuthaze ukuvutshelwa.
Iphakheji Nokulethwa
