Izengezo Zokudla Ezisezingeni Eliphezulu I-Lipase Enzyme CAS 9001-62-1 Umsebenzi We-enzyme Ye-Lipase Powder 100,000 u/g
Incazelo Yomkhiqizo
I-Lipase iwuhlobo lwe-enzyme eyi-catalytic ebandakanyeka kakhulu ekugayweni nasekugayweni kwamafutha emzimbeni. Nazi ezinye izici ezibalulekile zomzimba namakhemikhali e-lipase:
1.Izakhiwo zomzimba: Ama-Lipase ngokuvamile angamaprotheni angawodwa anesisindo samangqamuzana amakhulu uma kuqhathaniswa. Ivamise ukuncibilika emanzini futhi ingaba khona ngendlela emisiwe noma encibilikisiwe esigabeni esinamanzi. Izinga lokushisa elilungile lokusebenza le-lipase livamise ukuba ku-30-40°C, kodwa ezinye izinhlobo ezikhethekile ze-lipase zingasebenza emazingeni okushisa aphansi noma aphezulu.
2. Izici ze-Catalytic: Umsebenzi oyinhloko we-lipase ukugqugquzela ukusabela kwe-hydrolysis yamafutha. Iphula ama-triglycerides abe yi-glycerol nama-fatty acids, iphule izibopho ze-ester phakathi kwama-fatty acids ne-glycerol ngokungeza ama-molecule amanzi kuma-fatty esters. Ngaphezu kwalokho, i-Lipase ingakwazi futhi ukuvuselela ukusabela kwe-esterification kanye ne-transesterification ngaphansi kwezimo ezifana nama-surfactants.
3. Ukucaciswa kwe-substrate: I-Lipase inokucaciswa okuhlukile kwezinhlobo ezahlukene ze-lipid substrates. Ingenza i-hydrolysis yama-acids aphakathi nendawo namade kodwa ayisebenzi kakhulu ngokumelene nama-short-chain fatty acids. Ngaphezu kwalokho, i-lipase ingaphinda ihlanganise ama-lipid substrates ahlukahlukene njenge-triglycerides, phospholipids, ne-cholesterol esters.
4. Ithintwa izimo zemvelo: Umsebenzi we-Lipase we-catalytic uthintwa uchungechunge lwezimo zemvelo, njengezinga lokushisa, inani le-pH, ukugxiliswa kwe-ion, njll. Amazinga okushisa aphezulu namavelu e-pH afanelekile ngokuvamile akhuphula umsebenzi we-Lipase, kodwa phezulu kakhulu. noma amazinga okushisa aphansi kanye namanani e-pH angaholela ekwehliseni noma ekulahlekelweni okuphelele komsebenzi we-catalytic. Ngaphezu kwalokho, amanye ama-ion ensimbi afana ne-calcium ions nama-zinc ions angathuthukisa umsebenzi we-lipase. Ukufingqa, i-lipase iyi-enzyme enomsebenzi okhethekile we-catalytic ongadala ukusabela kwe-hydrolysis yamafutha. Umsebenzi wayo we-catalytic uthintwa uchungechunge lwezimo zemvelo futhi unokucaciswa okuthile kwama-substrates.Lezi zakhiwo zivumela i-lipase ukuthi idlale indima ebalulekile emzimbeni.
Umsebenzi
I-Lipase iyi-enzyme edlala indima ebalulekile ezintweni eziphilayo. Umsebenzi wawo oyinhloko ukusheshisa ukuwohloka nokugaya kwamafutha, ukudiliza ama-molecule amafutha abe ama-molecule amancane e-glycerol kanye ne-fatty acid. Lokhu kuvumela amafutha ukuthi amuncwa ngokuphumelelayo futhi asetshenziswe ngumzimba. Lokhu okulandelayo yimisebenzi esemqoka ye-Lipase:
1.Ukugaya kwamafutha: I-Lipase ikhishwa amanyikwe emzimbeni womuntu, futhi ibamba iqhaza ekuqhekekeni kwamafutha ohlelweni lokugaya ukudla. Lapho ukudla kunamafutha, amanyikwe akhulula i-Lipase emathunjini amancane. I-Lipase isebenza ngosawoti we-bile ku-bile ukuze ihlukanise ama-molecule wamafutha abe yi-glycerol nama-fatty acids. Lokhu kuvumela amafutha ukuba afakwe emathunjini amancane.
2.Ukumuncwa Komsoco: Ngokuphula ama-molecule wamafutha abe yi-glycerol encane nama-fatty acids, i-Lipase ithuthukisa ukunyibilika kwamafutha futhi ikhuthaze ukumuncwa kwamafutha emzimbeni. Amafutha angenye yemithombo ebalulekile yamandla emzimbeni futhi athwala amavithamini ancibilikayo (njengamavithamini A, D, E no-K), ngakho indima ye-Lipase ibalulekile ekumunceni izakhi zomsoco ngendlela efanele.
I-3.Ukulawulwa kwe-Metabolic: I-Lipase ayihileli nje kuphela ekuboleni nasekumunceni amafutha, kodwa futhi nasekulawuleni i-fat metabolism. Ilawula ukugcinwa nokukhululwa kwamafutha emzimbeni, ilawula isisindo somzimba kanye nokulinganisela kwamandla. Lapho umzimba udinga amandla, i-Lipase iyacushwa ukuze ikhiphe ama-fatty acids agcinwe kuma-fat cells ukuze asetshenziswe ngumzimba.
Ngamafuphi, i-Lipase idlala indima ebalulekile ohlelweni lokugaya ukudla komuntu. Ibamba iqhaza ekwakhiweni, ekugayeni nasekumunceni amafutha, futhi ilawula inqubo ye-metabolic yamafutha. Kubalulekile ekugayeni amafutha ngendlela efanele kanye nokumuncwa kwezakhi.
Isicelo
I-Lipase iyi-enzyme ye-lipolytic ephula ama-molecule wamafutha abe ama-fatty acids kanye ne-glycerol. Ngakho-ke, isetshenziswa kabanzi kulezi zimboni ezilandelayo:
1.Imboni Yokucubungula Ukudla: I-Lipase isetshenziswa njengesengezo ekucutshungulweni kokudla ukuze kuthuthukiswe ukunambitheka nokuthungwa kokudla. Ingasetshenziswa ekukhiqizeni imikhiqizo yobisi (njengoshizi, ibhotela, njll.) ukuthuthukisa ukunambitheka nokuthuthukisa ukunambitheka. Ngaphezu kwalokho, ingasetshenziswa ukukhiqiza ama-fat substitutes ukuze kuthuthukiswe impilo yokudla.
2.Imboni ye-Biofuel: I-Lipase isetshenziswa ekukhiqizeni i-biodiesel. Iguqula uwoyela ube yi-glycerol nama-fatty acids, ihlinzeke nge-feedstock yokwenza i-biodiesel.
3.Inkambu ye-Biotechnology: I-Lipase isetshenziswa kakhulu emkhakheni we-biotechnology. Ingasetshenziswa ezifundweni zaselabhorethri ze-fat metabolism kanye ne-fatty acid synthesis. Ngaphezu kwalokho, ama-lipase angasebenza njengengxenye ebalulekile yama-biosensors ukuze athole futhi alinganise okuqukethwe kwe-fatty acid.
I-4.Ukwenziwa Kwemithi: I-Lipase inezinhlelo zokusebenza ezihlukahlukene embonini yokukhiqiza imithi. Ingasetshenziselwa ukuhlanganiswa kwezidakamizwa kanye nezinqubo zokuhlanza, kanye nasekulungiseleleni izidakamizwa ze-lipid. Ngaphezu kwalokho, i-lipase ingasetshenziswa futhi ekwelapheni izifo zesistimu yokugaya ukudla, njenge-pancreatitis, isifo se-gallbladder, njll. njengendlela yokwelapha eyisizayo.
I-5.Imboni yokukhiqiza umkhiqizo wamakhemikhali wansuku zonke: I-Lipase ingasetshenziswa ku-detergents nemikhiqizo yokuhlanza ukusiza ukususa ama-grease nama-grease amabala nokuthuthukisa imiphumela yokuhlanza. Ingasetshenziswa futhi kuzimonyo kanye nemikhiqizo yokunakekelwa komuntu siqu ukuthuthukisa ukuthungwa nokunambitha kwemikhiqizo.
Kafushane, i-lipase idlala indima ebalulekile ekucutshungulweni kokudla, ama-biofuels, i-biotechnology, ukukhiqizwa kwemithi, imikhiqizo yamakhemikhali yansuku zonke nezinye izimboni. Izakhiwo zayo ze-lipolytic ziyenza ibe yi-enzyme ebalulekile ekwenzeni nasekucwaningeni kwemikhiqizo eminingi.
Imikhiqizo Ehlobene:
I-Newgreen factory iphinde inikeze ama-Enzyme njengokulandelayo:
Ibanga lokudla i-bromelain | I-Bromelain ≥ 100,000 u/g |
Ibanga lokudla le-alkaline protease | I-alkaline protease ≥ 200,000 u/g |
I-papain yebanga lokudla | I-Papain ≥ 100,000 u/g |
I-laccase yebanga lokudla | I-Laccase ≥ 10,000 u/L |
Uhlobo lokudla lwe-acid protease APRL | I-Acid protease ≥ 150,000 u/g |
I-Cellobiase yebanga lokudla | I-Cellobiase ≥1000 u/ml |
I-enzyme ye-dextran yebanga lokudla | I-Dextran enzyme ≥ 25,000 u/ml |
Ibanga lokudla lipase | I-Lipases ≥ 100,000 u/g |
Ukudla okungathathi hlangothi kwe-protease | I-protease emaphakathi ≥ 50,000 u/g |
I-glutamine transaminase yezinga lokudla | I-Glutamine transaminase≥1000 u/g |
Ibanga lokudla le-pectin lyase | I-Pectin lyase ≥600 u/ml |
I-pectinase yebanga lokudla (uketshezi 60K) | I-Pectinase ≥ 60,000 u/ml |
Ukudla grade catalase | I-Catalase ≥ 400,000 u/ml |
I-glucose oxidase yezinga lokudla | I-glucose oxidase ≥ 10,000 u/g |
Ibanga lokudla i-alpha-amylase (imelana namazinga okushisa aphezulu) | Izinga lokushisa eliphezulu α-amylase ≥ 150,000 u/ml |
Ibanga lokudla i-alpha-amylase (izinga lokushisa elimaphakathi) uhlobo lwe-AAL | Izinga lokushisa elimaphakathi i-alpha-amylase ≥3000 u/ml |
I-alpha-acetyllactate decarboxylase yezinga lokudla | I-α-acetyllactate decarboxylase ≥2000u/ml |
I-β-amylase yezinga lokudla (uketshezi 700,000) | β-amylase ≥ 700,000 u/ml |
Ibanga lokudla uhlobo lwe-β-glucanase BGS | β-glucanase ≥ 140,000 u/g |
I-protease yebanga lokudla (uhlobo lwe-endo-cut) | I-Protease (uhlobo lokusika) ≥25u/ml |
Uhlobo lwe-xylanase XYS lwebanga lokudla | I-Xylanase ≥ 280,000 u/g |
I-xylanase yebanga lokudla (i-acid 60K) | I-Xylanase ≥ 60,000 u/g |
Ibanga lokudla le-glucose amylase uhlobo lwe-GAL | I-enzyme yokugaya≥260,000 u/ml |
Ibanga lokudla i-Pullulanase (i-liquid 2000) | I-Pullulanase ≥2000 u/ml |
I-cellulase yebanga lokudla | I-CMC≥ 11,000 u/g |
I-cellulase yebanga lokudla (ingxenye egcwele engu-5000) | I-CMC≥5000 u/g |
I-alkaline protease yebanga lokudla (uhlobo olugxile emsebenzini omkhulu) | Umsebenzi we-alkaline protease ≥ 450,000 u/g |
I-glucose amylase yezinga lokudla (i-solid 100,000) | Umsebenzi we-glucose amylase ≥ 100,000 u/g |
I-acid grade protease (eqinile engu-50,000) | Umsebenzi we-Acid protease ≥ 50,000 u/g |
I-protease yebanga lokudla engathathi hlangothi (uhlobo olugxile emsebenzini omkhulu) | Umsebenzi we-protease omaphakathi ≥ 110,000 u/g |