Epimedium(Horny Goat Weed) Extract- Icariin e ba Tšepo e Ncha ea ho Loantša Kankere ea Urothelial

Urothelial carcinoma ke e 'ngoe ea mefuta e atileng haholo ea mofets'e, 'me ho hlaha ha hlahala le metastasis e le lisosa tse kholo tsa bokamoso. Ka 2023, ho hakanngoa hore linyeoe tse 168,560 tsa mofets'e oa moroto li tla fumanoa United States, 'me ho shoele batho ba ka bang 32,590; hoo e ka bang 50% ea linyeoe tsena ke urothelial carcinoma. Ho sa tsotellehe ho fumaneha ha mekhoa e mecha ea phekolo, e kang chemotherapy e thehiloeng platinum le PD1 antibody-based immunotherapy, bakuli ba fetang halofo ea urothelial carcinoma ba ntse ba sa arabele liphekolo tsena. Ka hona, ho na le tlhokahalo e potlakileng ea ho batlisisa mahlahana a macha a phekolo ho ntlafatsa boemo ba bakuli ba urothelial carcinoma.

Icariin(ICA), motsoako o ka sehloohong o sebetsang ho Epimedium, ke moriana oa setso oa Machaena oa tonic, aphrodisiac le anti-rheumatic. Hang ha e nkiloe, ICA e kenngoa ka metabolized ho icartin (ICT), e fanang ka liphello tsa eona. ICA e na le mesebetsi e mengata ea likokoana-hloko, ho kenyelletsa le ho laola ho itšireletsa mafung, ho ba le thepa ea antioxidant, le ho thibela tsoelo-pele ea hlahala. Ka 2022, li-capsules tsa Icaritin tse nang le ICT e le motsoako o ka sehloohong li ile tsa amoheloa ke Tsamaiso ea Sechaba ea Lihlahisoa tsa Bongaka ea Chaena (NMPA) bakeng sa phekolo ea pele ea kankere e tsoetseng pele e sa sebetseng ea hepatocellular carcinoma. Ho feta moo, e bontšitse katleho e kholo ea ho lelefatsa bophelo ka kakaretso ba bakuli ba nang le kankere e tsoetseng pele ea hepatocellular carcinoma. ICT ha e bolaee feela lihlahala ka ho toba ka ho etsa hore apoptosis le autophagy, empa hape e laola tumor immune microenvironment mme e khothalletsa karabelo ea 'mele ea ho itšireletsa mafung e khahlanong le hlahala. Leha ho le joalo, mokhoa o tobileng oo ICT e laolang TME ka oona, haholo-holo ka urothelial carcinoma, ha e utloisisoe ka botlalo.

Haufinyane tjena, bafuputsi ba Lefapha la Urology, Sepetlele sa Huashan, Univesithi ea Fudan e hatisitse sehlooho se reng "Icaritin e thibela tsoelo-pele ea mofetše oa urothelial ka ho hatella PADI2-mediated neutrophil infiltration le neutrophil extracellular trap formation" koranteng ea Acta Pharm Sin B. Phuputso e senotse Sin B. horeicariine fokolitse haholo ho ata ha hlahala le tsoelo-pele ha e ntse e thibela ho kenella ha neutrophil le NET synthesis, e bontšang hore ICT e ka 'na ea e-ba NETs inhibitor e ncha le phekolo e ncha ea urothelial carcinoma.

Ho khutla ha hlahala le metastasis ke tsona lisosa tse kholo tsa lefu ho urothelial carcinoma. Ka har'a tumor microenvironment, limolek'hule tse laolang hampe le li-multiple immune cell subtypes li hatella ho itšireletsa mafung. The inflammatory microenvironment, e amanang le neutrophils le neutrophil extracellular traps (NETs), e khothalletsa metastasis ea hlahala. Leha ho le joalo, hajoale ha ho na lithethefatsi tse thibelang ka ho khetheha li-neutrophils le NETs.

Phuputsong ena, bafuputsi ba bontšitse ka lekhetlo la pele seoicariin, phekolo ea pele bakeng sa kankere e tsoetseng pele le e sa phekoleheng ea hepatocellular carcinoma, e ka fokotsa li-NET tse bakoang ke ho ipolaea NETosis le ho thibela ho kenella ha neutrophil ka har'a tumor microenvironment. Ka mekhoa, ICT e tlama le ho thibela polelo ea PADI2 ho li-neutrophils, kahoo e thibela PADI2-mediated histone citrullination. Ho phaella moo, ICT e thibela tlhahiso ea ROS, e thibela tsela ea pontšo ea MAPK, 'me e hatella metastasis ea tumor e bakoang ke NET.

Ka nako e ts'oanang, ICT e thibela tumor PADI2-mediated histone citrullination, kahoo e thibela ho ngoloa ha liphatsa tsa lefutso tsa neutrophil tse kang GM-CSF le IL-6. Ka lehlakoreng le leng, ho theoleloa ha polelo ea IL-6 ho theha letoto la maikutlo a taolo ka JAK2/STAT3/IL-6 axis. Ka phuputso ea morao-rao ea lisampole tsa kliniki, bafuputsi ba fumane khokahano lipakeng tsa li-neutrophils, NETs, ​​prognosis ea UCa le phonyoha ea 'mele ea ho itšireletsa mafung. ICT e kopantsoeng le immune checkpoint inhibitors e ka ba le phello ea synergistic.

Ka kakaretso, thuto ena e fumane horeicariinhaholo fokotsa hlahala ho ata le tsoelo-pele ha a ntse a inhibiting neutrophil infiltration le NET synthesis, le neutrophils le NETs phetha karolo inhibitory ka hlahala immune microenvironment ea bakuli ba urothelial carcinoma. Ho feta moo, ICT e kopantsoeng le anti-PD1 immunotherapy e na le phello ea synergistic, e fana ka maikutlo a leano la phekolo bakeng sa bakuli ba urothelial carcinoma.