Phepelo ea fektheri E Neutral protease enzyme bakeng sa indasteri ea koae e fokotsang protheine ea lisakerete tsa makhasi
Tlhaloso ea Sehlahisoa
Protease e sa nke lehlakore e hlahisoa ke Bacillus subtilis ka ho belisoa ha metsi a tebileng, ultrafiltration le mekhoa e meng.E ka etsa hore hydrolysis ea protheine e hlahise li-amino acid le li-peptide tse sa lefelloeng sebakeng se se nang lehlakore kapa se fokolang kapa tikoloho ea alkaline. Ka lebaka la melemo ea lebelo le phahameng la catalytic reaction, maemo a bonolo le taolo e bonolo ea karabelo, protease e sa nke lehlakore e sebelisitsoe haholo indastering.
Mosebetsi
1. Ho eketsa protease ho bolisa protheine e mahlakung a koae ho ka fokotsa boleng ba koae e tukang, ho fokotsa bohale, ho teneha le tatso e babang, le ho ntlafatsa boemo ba makhasi a koae.
2. E ka ntlafatsa monko oa koae ka katleho, ea ntlafatsa sebopeho sa ho tsuba, le ho fokotsa tatso ea tlhaho ea coke le khase e fapaneng, e le hore monko o monate o be betere, 'me o khone ho hokahanya moea oa mosi, ho fokotsa tatso ea coke.
3.Lik'hemik'hale tse ka hare tsa makhasi a koae li lumellana haholoanyane 'me boleng ba kutlo ba makhasi a koae bo ntlafatsoa.
Mokhoa oa Kopo
Tekanyetso ea enzyme: tekanyetso e khothaletsoang ka kakaretso ke 0.01-3kg ea ho lokisoa ha enzyme ka tone e le 'ngoe ea lisebelisoa tse tala.Tšoa kutu ea makhasi a koae 'me u a khaole ka maqephe; lekanya tekanyo e itseng ea protease ho lokisa motsoako o itseng oa tharollo. Ha ho sebelisoa palo, tekanyo e itseng ea tharollo ea enzyme e ile ea lekanyetsoa le ho fafatsoa ka ho lekana makhasing a liteko a koae ka thepa e iketselitseng ea ho iphepa. Makhasi a koae a ile a kenngoa ka kamoreng e sa khaotseng ea mocheso le mongobo bakeng sa enzymatic hydrolysis tlas'a maemo a behiloeng a liteko.
Makhasi a koae a phekotsoeng a ne a sa sebetse ho 120 ℃, a khaoloa likotoana ebe a behoa ka thoko. Ka lebaka la phapang ea tšimo ea kopo le sebopeho sa thepa e tala le li-parameter tsa ts'ebetso ea fektheri e 'ngoe le e' ngoe, mokhoa oa 'nete oa ho eketsa le palo ea sehlahisoa sena e lokela ho khethoa ka tlhahlobo.
Polokelo
| E hantle pele ho | Ha e bolokiloe ka mokhoa o khothalelitsoeng, sehlahisoa se sebelisoa hamolemo nakong ea likhoeli tse 12 ho tloha letsatsing la ho pepa. |
| Polokelo ho | 0-15 ℃ |
| Maemo a polokelo | Sehlahisoa sena se lokela ho bolokoa sebakeng se pholileng le se omileng ka setshelo se tiisitsoeng, ho qoba ho insolation, mocheso o phahameng le mongobo. Sehlahisoa se entsoe bakeng sa botsitso bo nepahetseng. Polokelo e atolositsoeng kapa maemo a mabe joalo ka mocheso o phahameng kapa mongobo o phahameng a ka lebisa tlhokong e phahameng ea litekanyetso. |
Lihlahisoa tse Amanang:
Feme ea Newgreen e boetse e fana ka li-Enzymes ka tsela e latelang:
| Bromelain ea boemo ba lijo | Bromelain ≥ 100,000 u / g |
| Mokhahlelo oa lijo oa alkaline protease | Alkaline protease ≥ 200,000 u / g |
| Papain ea boemo ba lijo | Papain ≥ 100,000 u / g |
| Lijo tsa boemo ba laccase | Laccase ≥ 10,000 u/L |
| Mofuta oa acid oa protease oa mofuta oa APRL | Acid protease ≥ 150,000 u / g |
| Cellobiase ea boemo ba lijo | Cellobiase ≥1000 u / ml |
| Li-enzyme tsa dextran tsa boemo ba lijo | Dextran enzyme ≥ 25,000 u / ml |
| Lipase tsa boemo ba lijo | Lipase ≥ 100,000 u / g |
| Protease ea boemo ba lijo | Protease e sa nke lehlakore ≥ 50,000 u / g |
| Mofuta oa lijo oa glutamine transaminase | Glutamine transaminase≥1000 u/g |
| Mofuta oa lijo tsa pectin lyase | Pectin lyase ≥600 u / ml |
| Pectinase ea boemo ba lijo (metsi a 60K) | Pectinase ≥ 60,000 u / ml |
| Catalase ea boemo ba lijo | Catalase ≥ 400,000 u / ml |
| Sekhahla sa glucose oxidase | Glucose oxidase ≥ 10,000 u / g |
| Sehlopha sa lijo sa alpha-amylase (e hanana le mocheso o phahameng) | Mocheso o phahameng oa α-amylase ≥ 150,000 u / ml |
| Sehlopha sa lijo sa alpha-amylase (mocheso o mahareng) Mofuta oa AAL | Mocheso o mahareng alpha-amylase ≥3000 u / ml |
| Alpha-acetyllactate decarboxylase ea boemo ba lijo | α-acetyllactate decarboxylase ≥2000u/ml |
| Boemo ba lijo β-amylase (metsi 700,000) | β-amylase ≥ 700,000 u / ml |
| Mofuta oa lijo oa mofuta oa β-glucanase BGS | β-glucanase ≥ 140,000 u/g |
| Protease ea boemo ba lijo (mofuta oa endo-cut) | Protease (mofuta o sehiloeng) ≥25u/ml |
| Mofuta oa lijo oa xylanase XYS | Xylanase ≥ 280,000 u / g |
| xylanase ea boemo ba lijo (acid 60K) | Xylanase ≥ 60,000 u / g |
| Mofuta oa GAL oa mofuta oa glucose amylase | Enzyme ea tlhaho≥260,000 u/ml |
| Mokhahlelo oa lijo Pullulanase (metsi a 2000) | Pullulanase ≥2000 u / ml |
| Cellulase ea boemo ba lijo | CMC≥ 11,000 u/g |
| Cellulase ea boemo ba lijo (karolo e felletseng ea 5000) | CMC≥5000 u/g |
| Alkaline protease ea boemo ba lijo (mofuta o nang le ts'ebetso e phahameng) | Mosebetsi oa alkaline protease ≥ 450,000 u / g |
| Glucose amylase ea boemo ba lijo (100,000 e tiileng) | Mosebetsi oa Glucose amylase ≥ 100,000 u / g |
| Protease ea boemo ba lijo (e tiileng 50,000) | Mosebetsi oa protease ea acid ≥ 50,000 u / g |
| Protease ea boemo ba lijo e sa nke lehlakore (mofuta o nang le ts'ebetso e phahameng) | Ts'ebetso ea protease e sa nke lehlakore ≥ 110,000 u / g |
tikoloho ea feme
sephutheloana & thomello
sepalangoang
