China na-ebunye Amylase-nri Alfa Amylase ọnụahịa Enzyme na-eguzogide okpomọkụ
Nkọwa ngwaahịa
Okwu mmalite α-amylase dị elu
α-amylase okpomọkụ dị elu bụ enzyme dị mkpa a na-ejikarị eme ihe maka hydrolysis nke starch. Ọ nwere ike mebie ire ere starch n'okpuru ọnọdụ okpomọkụ dị elu iji mepụta maltose, glucose na oligosaccharides ndị ọzọ. Nke a bụ ụfọdụ isi ihe gbasara alpha-amylase okpomọkụ:
1. Isi mmalite
A na-enwetakarị alpha-amylase okpomọkụ dị elu site na ụfọdụ microorganisms (dị ka nje bacteria na fungi), karịsịa thermophiles (dị ka Streptomyces thermophilus na Bacillus thermophilus ), microorganisms ndị a nwere ike ịdị ndụ na gburugburu okpomọkụ dị elu ma mepụta enzyme a.
2. Atụmatụ
- Nguzogide okpomọkụ dị elu: Alfa-amylase dị elu nwere ike ịnọgide na-arụ ọrụ na okpomọkụ dị elu (na-emekarị 60 ° C ruo 100 ° C) ma dị mma maka nhazi okpomọkụ dị elu.
- Ngbanwe pH: na-arụkarị ọrụ kacha mma n'okpuru ọnọdụ na-anọpụ iche ma ọ bụ ntakịrị acidic, mana pH dị iche iche dịgasị iche dabere na isi iyi nke enzyme ahụ.
3. Nchekwa
A na-ewere alfa-amylase okpomọkụ dị elu dịka nchekwa, na-agbaso ụkpụrụ ndị dị mkpa maka mgbakwunye nri, ma na-ejikarị ya na ụlọ ọrụ nri.
Na nkenke, α-amylase okpomọkụ dị elu bụ enzyme dị mkpa nke nwere atụmanya ngwa sara mbara ma nwee ike imeziwanye nrụpụta starch nke ọma na ịdị mma ngwaahịa.
COA
| Ihe | Nkọwapụta | Nsonaazụ |
| Ọdịdị | N'efu na-eru nke ìhè odo siri ike ntụ ntụ | Na-akwado |
| Isi | Njirimara njirimara nke ísì gbaa ụka | Na-akwado |
| Nha ntupu/Sieve | NLT 98% Site na mesh 80 | 100% |
| Ọrụ nke enzyme (Alfa Amylase Enzyme) | 15,000 u/ml | Na-akwado |
| PH | 57 | 6.0 |
| Ọnwụ na ihicha | 5 ppm | Na-akwado |
| Pb | 3 ppm | Na-akwado |
| Ngụkọta ọnụ ọgụgụ efere | 50000 CFU/g | 13000CFU/g |
| E.Coli | Ihe ọjọọ | Na-akwado |
| Salmonella | Ihe ọjọọ | Na-akwado |
| Enweghi ike | ≤0.1% | tozuru oke |
| Nchekwa | A na-echekwa ya na akpa poly siri ike, na ebe dị mma na nke kpọrọ nkụ | |
| Ndụ nchekwa | Afọ 2 mgbe echekwara nke ọma | |
Ọrụ
Okpomọkụ α-amylase dị elu bụ enzyme dị mkpa a na-ejikarị eme ihe na ụlọ ọrụ nri na mpaghara ndị ọzọ. Isi ọrụ ya gụnyere:
1. Starch hydrolysis
- Catalysis: α-amylase dị elu nwere ike ime ka hydrolysis nke starch dị n'okpuru ọnọdụ okpomọkụ dị elu, na-agbaji starch n'ime obere mkpụrụ ndụ shuga, dị ka maltose na glucose. Usoro a dị oke mkpa maka iji starch eme ihe.
2. Melite arụmọrụ saccharification
- Usoro saccharification: N'ime nhazi na usoro saccharification, okpomọkụ dị elu α-amylase nwere ike imeziwanye arụmọrụ saccharification nke starch nke ọma, kwalite usoro ịgba ụka, na ịbawanye mmepụta mmanya ma ọ bụ ngwaahịa ndị ọzọ fermented.
3. Meziwanye udidi nri
- Nhazi mgwakota agwa: N'oge a na-eme achịcha, iji alpha-amylase na-ekpo ọkụ na-ekpo ọkụ na-eme ka ọ dịkwuo mma, na-eme ka ọ dịkwuo mma ma na-eme ka uto na ọdịdị nke ngwaahịa ahụ gwụchara.
4. Melite nkwụsi ike nke okpomọkụ
- Nguzogide okpomọkụ dị elu: α-amylase dị elu ka nwere ike ịnọgide na-arụ ọrụ na okpomọkụ dị elu ma dị mma maka nri a na-edozi na okpomọkụ dị elu, dị ka nri mkpọ na nri dị njikere iri nri.
5. Ngwa maka ụlọ ọrụ
- Biofuels: Na mmepụta biofuel, a na-eji alpha-amylase okpomọkụ dị elu mee ihe iji gbanwee starch ka ọ bụrụ sugar fermentable iji nye akụrụngwa maka mmepụta bioethanol.
- Textile na Akwụkwọ: N'ime ụlọ ọrụ textile na akwụkwọ, a na-eji alpha-amylase wepụ ihe mkpuchi starch ma melite ogo ngwaahịa.
6. Belata viscosity
- Mmelite nke mmiri mmiri: N'ime ụfọdụ nhazi nri, okpomọkụ dị elu α-amylase nwere ike belata viscosity nke starch slurry ma melite mmiri mmiri n'oge nhazi.
Na nkenke, α-amylase okpomọkụ dị elu na-arụ ọrụ dị mkpa n'ọtụtụ mpaghara ma nwee ike melite arụmọrụ starch na nhazi nke nri.
Ngwa
Ngwa nke elu okpomọkụ alfa-amylase
Okpomọkụ α-amylase nwere nnukwu ngwa n'ọtụtụ ụlọ ọrụ, ọkachasị gụnyere akụkụ ndị a:
1. Ime biya ụlọ ọrụ
- Mmepụta Beer: N'ime usoro ịme biya, a na-eji alpha-amylase okpomọkụ dị elu mee ihe iji gbanwee starch ka ọ bụrụ sugars fermentable, kwalite gbaa ụka, na ịbawanye mmepụta mmanya.
- Ihe ọṅụṅụ ndị ọzọ fermented: Ọ na-ekerekwa òkè yiri nke ahụ na mmepụta nke ihe ọṅụṅụ ndị ọzọ fermented.
2. Nhazi nri
- Usoro Saccharification: Na mmepụta nke swiiti, ihe ọṅụṅụ na ihe oriri ndị ọzọ, ọ na-enyere aka ịgbanwe starch n'ime shuga ma na-eme ka ụtọ na uto nke ngwaahịa ahụ dịkwuo mma.
- Achịcha na achịcha dị iche iche: N'oge a na-eme achịcha, na-eme ka mmiri na-arụ ọrụ nke mgwakota agwa na-eme ka ọ dịkwuo mma, ma kwalite ọdịdị na uto nke ngwaahịa emechara.
3. Biofuels
- Mmepụta Ethanol: Na mmepụta nke biofuels, a na-eji alpha-amylase okpomọkụ dị elu mee ihe iji gbanwee starch ka ọ bụrụ sugar fermentable iji nye akụrụngwa maka mmepụta nke bioethanol.
4. Akwa na akwụkwọ
- Mwepụ nke mkpuchi starch: N'ime ụlọ ọrụ textile na akwụkwọ, a na-eji alpha-amylase wepụ ihe mkpuchi starch iji melite ogo ngwaahịa na nhazi nhazi.
5. Ụlọ ọrụ nri
- Ihe mgbakwunye nri: Na nri anụmanụ, ịgbakwunye α-amylase okpomọkụ dị elu nwere ike imeziwanye nri nri ma kwalite uto anụmanụ.
6. Ihe ịchọ mma na ọgwụ
- Mmụba Efrata: Na ụfọdụ ihe ịchọ mma na ọgwụ ọgwụ, a na-eji alpha-amylase okpomọkụ dị elu mee ihe iji meziwanye ọdịdị na nkwụsi ike nke ngwaahịa ahụ.
Nchịkọta
Okpomọkụ α-amylase dị elu na-arụ ọrụ dị mkpa n'ọtụtụ mpaghara dị ka ịme ihe, nhazi nri, biofuels, textiles, na nri n'ihi nkwụsi ike ya na arụmọrụ ya na okpomọkụ dị elu.
Ngwungwu & Nbunye
